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Highly efficient over-production inE. coliof YvcC, a multidrug-like ATP-binding cassette transporter fromBacillus subtilis

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
0005-2736
Publisher
Elsevier
Publication Date
Volume
1565
Issue
1
Identifiers
DOI: 10.1016/s0005-2736(02)00515-1
Keywords
  • Yvcc
  • Atp-Binding Cassette
  • Bacillus Subtilis
Disciplines
  • Biology

Abstract

Abstract ATP-binding cassette (ABC) transporters have often been refractory to over-expression. Using the C41(DE3) E. coli as a host strain, membrane vesicles highly enriched (>50%) in YvcC, a previously uncharacterized ABC transporter from Bacillus subtilis homologous to P-glycoprotein multidrug transporters, were obtained. The functionality of YvcC was assessed by its high vanadate-sensitive ATPase activity and its ability to transport a fluorescent drug, the Hoechst 33342.

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