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Electron microscopical studies of phage multiplication:III. Observation of single cell bursts

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
3
Issue
2
Identifiers
DOI: 10.1016/0042-6822(57)90093-4

Abstract

Abstract The experimental conditions, under which the collodion agar filtration technique allows the electron microscopical observation of the products of lysed single cells without disturbing the development of the induced or infected cells are determined. The test used is that single bursts observed in the electron microscope give the same yield as measured in single bursts assayed by plate counts. The method, applied to T2-infected cells lysing after proflavine action, shows that every single cell produces empty heads and rods. By means of chloroform and ammonia vapors, premature lysis can be produced for observation in the electron microscope.

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