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Development of rapid detection system for allergic reaction using rat basophilic leukemia (RBL-1) cells

Authors
Journal
Sensors and Actuators B Chemical
0925-4005
Publisher
Elsevier
Publication Date
Volume
13
Identifiers
DOI: 10.1016/0925-4005(93)85389-r
Disciplines
  • Chemistry

Abstract

Abstract We have investigated the electrochemical detection system of allergic reaction using rat basophilic leukemia (RBL-1) cells by applying cyclic voltammetry. When the potential of the electrode is run in the range 0—1.0 V versus a saturated calomel electrode (SCE), an anodic peak current appears around 0.34 V. This peak current or RBL-1 cells is attributed to intracellular serotonin. After the addition of allergen, this peak current increases with time and the maximum current (0.82 μA per 2×10 5 cells) is obtained after 20 min. On the other hand, the current is constant when the allergen is not added. This current increase is attributed to localization of the intracellular serotonin and is specific for allergen.

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