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A simple method for the production of migration inhibitory factor by concanavalin A-stimulated lymphocytes

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
14
Issue
2
Identifiers
DOI: 10.1016/0022-1759(77)90004-7

Abstract

Abstract A simple method for preparing culture supernatants containing macrophage migration inhibitory factor (MIF) by pulse exposure of guinea pig lymphocytes to concanavalin A (Con A), is described. The method is based on the property of originally nonadherent lymphocytes to attach to the flask surface in the presence of Con A. The Con A-induced lymphocyte ‘monolayer’ can be easily rinsed, allowing the removal of free Con A without repeated, cell damaging centrifugation. The Con A-pulsed lymphocytes are cultured for 24 h in medium and supernatants containing MIF but free of undesired Con A are regularly obtained.

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