Affordable Access

Publisher Website

HPLC analysis of antioxidants

Authors
Journal
Talanta
0039-9140
Publisher
Elsevier
Publication Date
Volume
37
Issue
3
Identifiers
DOI: 10.1016/0039-9140(90)80057-m
Disciplines
  • Chemistry
  • Physics

Abstract

Abstract Qualitative and quantitative HPLC methods are described for the analysis of mixtures of twelve antioxidants. For identification of the components present, gradient elution with a convex profile from 35:65 v/v water-methanol to pure methanol is used, on a Waters 5 μm C 18 Resolve column, with an ultraviolet detector. Propyl gallate and methyl p-hydroxybenzoate cannot be separated, however. For quantitative analysis, with ultraviolet and electrochemical detectors in series, the 35:65 water-methanol mixture or pure methanol is used as the eluent, under isocratic conditions, with lithium perchlorate as supporting electrolyte. An applied potential ranging from +0.8 to + 1.7 V allows detection of all the antioxidants tested. Both modes of detection are very sensitive, with limits of detection as low as 61 pg (UV, methyl p-hydroxybenzoate) and 360 pg (electrochemistry, butylhydroxyanisole).

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

HPLC analysis of antioxidants.

on Talanta March 1990

Stoichiometric analysis by chromatographie techniq...

on International Journal of Pharm... Jan 01, 1982

Qualitative and quantitative analysis of the major...

on Journal of the Science of Food... Aug 15, 2012
More articles like this..