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Replacement of three troponin components with cardiac troponin components within single glycerinated skeletal muscle fibers

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
181
Issue
3
Identifiers
DOI: 10.1016/0006-291x(91)92039-m

Abstract

Abstract The tension of single glycerinated rabbit skeletal muscle fiber was desensitized to a Ca 2+-concentration after treatment with an excessive amount of bovine cardiac troponin T and reached a level of about 70% of the maximum tension of the untreated fiber. A SDS-gel electrophoretic examination indicated that troponin C·I·T complex in the fiber was replaced with the added cardiac troponin T. The Ca 2+-sensitivity of the tension of the troponin T-treated fiber was then recovered by the addition of bovine cardiac troponins I and C. The rabbit skeletal muscle fiber thus hybridized with bovine cardiac troponin C·I·T showed the same cooperativity of Ca 2+-activation as the cardiac muscle.

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