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Dual parameter, quantitative cytofluorometric analysis of endogenous H-2Kkand foreign HLA class I molecules expressed at the surface of murine transformed L cells

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
149
Issue
2
Identifiers
DOI: 10.1016/0014-4827(83)90370-1

Abstract

Abstract By using a calibrated dual laser cell sorter and monoclonal antibodies directly conjugated to fluorescein and rhodamine and specific for H-2K k and HLA class I antigens, quantitative cytofluorometric analysis was performed on individual HLA-A3 or -CW3 transformed mouse L cells (H-2 k). More than 80% of these cells expressed both HLA class I and H-2K k molecules. Their respective levels of expression were calculated: a mean of 4 × 10 5 HLA class I and 2.3 × 10 5 H-2K k molecules per single cell. Quantitative comparison with control untransformed L cells and double fluorescence contour maps showed a positive correlation between the levels of expression of HLA class I and H-2K k molecules suggesting that expression of foreign class I molecules did not occur at the expense of the endogenous H-2 k product.

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