Affordable Access

Publisher Website

Development of an assay for penicillin G in chinook salmon muscle tissue

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
626
Issue
2
Identifiers
DOI: 10.1016/0021-9673(92)85420-x
Disciplines
  • Physics

Abstract

Abstract A high-performance liquid chromatographic method was developed to assay penicillin G (pen-G) in chinook salmon muscle tissue. Solid-phase extraction techniques were used to recover pen-G and the internal standard, phenoxymethylpenicillin, from the salmon tissue samples. Pen-G was analyzed using a 25 cm × 4.6 mm I.D. Ultrasphere OS (C 8) column and a mobile phase of acetonitrile—0.02 M phosphate buffer, pH 6.75 (43:200) with ultraviolet detection at 214 nm. A linear calibration curve ( r 2 = 0.9994) was obtained for pen-G over the range 0.05–3.0 ppm. Recoveries of pen-G added to tissues at 0.1, 1.0 and 2.0 ppm were 63.9%, 64.2% and 65.4%, respectively, in salmon muscle tissue, and intra-assay variabilities of 4.89% at 1.0 ppm and 2.79% at 2.0 ppm were optimized for salmon muscle tissue. The detection limit of pen-G was 0.05 ppm in salmon muscle tissue, using a signal-to-noise ratio of 5:1.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Penicillin residues in treated Chinook salmon

on Food Research International Jan 01, 1994

Accumulation of butyltins in muscle tissue of chin...

on Marine Pollution Bulletin Jan 01, 1986

High-performance liquid chromatographic determinat...

on Journal of Chromatography A Jan 01, 1993
More articles like this..