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Detection of the tripeptide Tyr-Gly-Gly, a putative enkephalin metabolite in brain, using a sensitive radioimmunoassay

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
189
Issue
2
Identifiers
DOI: 10.1016/0014-5793(85)81049-8
Keywords
  • Enkephalin Metabolism
  • Tyr-Gly-Gly
  • Radioimmunoassay

Abstract

Abstract A sensitive and specific radioimmunoassay has been developed for YOG. The tripeptide was previously derivatised with p-benzoquinone to prepare the immunogen and the 125 tracer as well as in samples submitted to the RIA. The sensitivity is about 1 nM as compared with 8000 nM for underivatised YGG. Measurable amounts of endogenous YGG immunoreactivity, co-eluting in HPLC with authentic YGG, were detected in mouse striatal extracts.

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