Increasingly is the heat of the soil used like a renewable energy source, when low potential energy of the land is transferred by a heat pump to higher level and like this is used for heating buildings. When optimizing the parametrs of the proposed thermal collectors is necessary to know the thermal properties of soils. The experimental part of the submitted thesis therefore deals with the measurement and assessing the values of thermal conductivity, heat capacity, thermal duffusivity and thermal resistance, depending on volumetric water content and other soil properties for selected soil types and substrates. For measuring was selected 11 soil types, for which was the soil sample taken from horizon A, and 2 soil substrates (sand and loess), totaly its soil samples from 13 different locations in Czech Republic. For each soil was ever taken two samples for measuring, using a apparatus KD2 Pro and his two sensors, the TR-1 and the SH-1. The value for thermal conductivity, heat capacity, thermal diffusivity and thermal resistance was measured for various humidity. The measured data were processed into graphs and than analyzed. The dependence of all measured characteristics on volume water content was proven. The thermal conductivity increases with increasing volume water content, while the thermal resistivity decrease with increasing volume water content, volume heat capacity increase with increasing volume water content as well as thermal diffusivity increase with increasing water content. But there were also other proven addictions, especially on the content of sand, organic matter and silt, which differ in each soil. The thermal conductivity reaches considerably higher values in soils with higher sand content, even though that these soil are not saturated with water like other, which are less in sand content. In case of volumetric heat capacity is dependency on volume water content directly proportional. Higher values were achieved in soils with higher content of organic matter and lower content of sand, which is unable to absorb large amount of water.