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High-Throughput Isolation and Mapping of C. elegans Mutants Susceptible to Pathogen Infection

Authors
Journal
PLoS ONE
1932-6203
Publisher
Public Library of Science
Publication Date
Volume
3
Issue
8
Identifiers
DOI: 10.1371/journal.pone.0002882
Keywords
  • Research Article
  • Genetics And Genomics
  • Genetics And Genomics/Disease Models
  • Immunology/Innate Immunity
  • Infectious Diseases/Bacterial Infections

Abstract

We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor® as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5–8 megabase sub-chromosomal region.

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