Affordable Access

Benzoxazinone kanamycin A conjugate. A new fluorescent probe suitable to detect mycoplasmas in cell culture

Biology of the Cell
Wiley Blackwell (Blackwell Publishing)
Publication Date
  • Flow Cytometry
  • Polycation
  • Aminoglycoside


Summary The synthesis of a new benzoxazinone derivative suitable to detect early infection of cultured cells with mycoplasmas is described. p-[ β-(7- dimethylamino 1,4- benzoxazin 2-one 3yl) -vinyl] -phenylpropenoic acid was coupled to kanamycin A, an aminoglycoside leading to a cationic fluorescent probe which fluoresces at 600 nm upon excitation at 490 nm. This fluorescent probe is shown to heavily label the glycocallix of all the mycoplasma strains tested which are found to be associated with contaminated cultured cells and to allow an easy and rapid detection of contamination by fluorescence microscopy and flow cytometry.

There are no comments yet on this publication. Be the first to share your thoughts.