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Production of fungal fructosyl amino acid oxidase useful for diabetic diagnosis in the peroxisome ofCandida boidinii

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
459
Issue
2
Identifiers
DOI: 10.1016/s0014-5793(99)01245-4
Keywords
  • Glycation
  • Diabetes
  • Diagnosis
  • Peroxisome
  • Methylotrophic Yeast
  • Gene Expression
Disciplines
  • Biology

Abstract

Abstract A high-level production of fructosyl amino acid oxidase (FAOD), whose production was toxic in Escherichia coli, was investigated through attempts to utilize the peroxisome of Candida boidinii as the place for protein accumulation. The alcohol oxidase-depleted strain (strain aod1Δ) produced FAOD at a four to five times higher level than the wild type strain in terms of protein amount and enzyme activity, although the transcriptional level was similar. As a result of this study, we could improve FAOD productivity approximately 47-fold from the original transformant, and FAOD accumulated within membrane-bound peroxisomes up to 18% of the total soluble proteins.

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