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Glycogen metabolism: a13C-NMR study on the isolated perfused rat heart

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
178
Issue
1
Identifiers
DOI: 10.1016/0014-5793(84)81234-x
Keywords
  • Perfused Rat Heart
  • 13C-Nmr
  • D-[1-13C]Glucose
  • Glycogen Metabolism

Abstract

Abstract Glycogen synthesis from D-[1- 13C]glucose was observed in the perfused rat heart by 13C-NMR spectroscopy at 62.9 MHz. The glycogenogenesis was stimulated by pretreatment of the animals with isoprenaline. Whereas in hearts from control rats the incorporation of D-[1- 13C]glucose into the glycogen remained below the detection threshold, 5 min proton-decoupled 13C-NMR spectra revealed, in hearts from treated rats, a significant labelling of the glycogen within the first minutes of the perfusion and a further linear increase of the glycogen resonance for up to 25 min. This model was used to monitor the appearance of 13C-labelled lactate during ischemia.

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