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Introduction of 5′-terminal functional groups into synthetic oligonucleotides for selective immobilization

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
164
Issue
2
Identifiers
DOI: 10.1016/0003-2697(87)90502-1
Keywords
  • Affinity Chromatography
  • Hplc
  • Nucleic Acids
  • Recombinant Technology
  • Nucleic Acid Chemistry
  • Organic Synthesis
  • Oligonucleotide Synthesis
Disciplines
  • Chemistry

Abstract

Abstract Oligodeoxyribonucleotides terminating in a 5′-primary amine group are synthesized using solid-phase supported phosphoramidite chemistry. The 5′-terminal amine group in the deprotected oligomers is further derivatized with either succinic anhydride to give 5′-carboxylic acid or with dithiobis(succinimidylpropionate) followed by treatment with dithioerythritol to produce 5′-thiol-terminated oligonucleotides. The 5′-thiol-terminated oligonucleotides are selectively immobilized on solid supports containing either p-chloromercuribenzoate or 2,2′-dithiobis(5-nitropyridine) activated thiol groups.

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