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In Vitro Regeneration of Brinjal (Solanum melongena L.) Using Stem and Leaf Explants

Institute of Biological Sciences, Rajshahi University
Publication Date
  • Biotechnology
  • Brinjal
  • Engineering
  • Medicine


Antibacterial activity and cytotoxicity study of the lectins purified from Sajna (Moringa oleifera L J. bio-sci. 17: 155-158, 2009 ISSN 1023-8654 -Short Communication IN VITRO REGENERATION OF BRINJAL (SOLANUM MELONGENA L.) USING STEM AND LEAF EXPLANTS B P Ray*, L Hassan, S K Sarker Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh Brinjal (Solanum melongena L.), belongs to the family Solanaceae, is one of the most popular, palatable and nutritious vegetable crop in Bangladesh. Brinjal is highly susceptible to different insects, pests and diseases that exert a deleterious effect on yield, market quality, storability and international germplasm distribution. The seed-borne pathogens of previous year can be perpetuated over the generations with symptoms expressed. To overcome this situation plant tissue culture offers an efficient method for pathogen free materials and germplasm preservation of plants. The regeneration of plants from cell and tissue culture is an important and essential component of biotechnology that is required for the genetic manipulation of plants. High frequency regeneration of plants from in vitro cultured tissues and cells are a pre-requisite for successful application of tissue culture and genetic engineering technologies for crop improvement. Culture of explants produces calli that are suitable materials for genetic transformation. Many attempts have been made to enhance the frequency of plant regeneration from brinjal callus and a lot of research programmes have been devoted to investigate the factors affecting plant regeneration. Healthy seeds of brinjal cv. Jhumki were collected from Bangladesh Agricultural Research Institute (BARI). The seeds were then washed thoroughly in running tap water. Special care was taken to avoid all types of injury. The surface sterilization of these seeds was carried out under a Laminar Air Flow Cabinet. The

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