Affordable Access

Publisher Website

Isolation of HIV-1 Protease-Inhibiting Peptides from Thermolysin Hydrolysate of Oyster Proteins

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
253
Issue
3
Identifiers
DOI: 10.1006/bbrc.1998.9824
Disciplines
  • Biology

Abstract

Abstract The peptides inhibiting HIV-1 protease were isolated from the hydrolysate of oyster ( Crassostrea gigas) proteins prepared with thermolysin. The amino acid sequences of the peptides were determined as Leu-Leu-Glu-Tyr-Ser-Ile and Leu-Leu-Glu-Tyr-Ser-Leu. These sequences exist in some proteins of variola major virus or human cytomegalovirus. Chemically synthesized Leu-Leu-Glu-Tyr-Ser-Ile and Leu-Leu-Glu-Tyr-Ser-Leu showed IC 50values of 20 and 15 nM, respectively, and behaved as competitive inhibitors for HIV-1 protease with K i values of 13 and 10 nM, respectively. These peptides were more potent as an HIV-1 protease inhibitor than pepstatin A.

There are no comments yet on this publication. Be the first to share your thoughts.