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Determination of relative antigen-antibody avidities by radial immunodiffusion

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
61
Issue
1
Identifiers
DOI: 10.1016/0022-1759(83)90013-3
Keywords
  • Radial Immunodiffusion
  • Antigen-Antibody Interaction
  • Quantitative Immunoelectrophoresis

Abstract

Abstract Radial immunodiffusion can be used to determine relative antigen-antibody avidities in exactly the same way as demonstrated previously for quantitative immunoelectrophoresis (Birkmeyer et al., 1981). Antigen-antibody interactions of greater avidity result in greater value of (Δ Area/Δ [Antigen]) in plots of immunoprecipitin circle area versus antigen concentration while interactions of equal avidity will yield equal values of (Δ Area/Δ [Antigen]). This was demonstrated using antigens of different weight ranging from 8000 to 66,000.

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