Affordable Access

Publisher Website

Enhancement by serotonin of GABA-mediated inhibitory synaptic currents in rat cerebellar Purkinje cells

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
173
Identifiers
DOI: 10.1016/0304-3940(94)90165-1
Keywords
  • Cerebellum
  • Purkinje Cell
  • Serotonin
  • γ-Aminobutyric Acid
  • Inhibitory Postsynaptic Current
  • Whole-Cell Voltage-Clamp Recording

Abstract

Abstract The actions of serotonin (5-HT) on synaptic responses in Purkinje cells were examined using the whole-cell voltage-clamp recording from thin slices of the rat cerebellum. Focal stimulation within the molecular layer elicited synaptic responses: (1) inhibitory postsynaptic currents (IPSCs) that were completely blocked by bicuculline; (2) excitatory postsynaptic currents (EPSCs) that were antagonized by CNQX; and (3) combination of IPSC and EPSC. Application of 5-HT (3–30 μM) increased the amplitude of the stimulation-evoked IPSCs but did not significantly affect the EPSCs. The frequency and the mean amplitude of spontaneous IPSCs were increased in the presence of 5-HT. Neither the sensitivity to exogenously applied GABA nor the holding current of Purkinje cells were noticeably altered by 5-HT. The results indicate that 5-HT differentially enhances the GABA-mediated IPSCs in rat cerebellar Purkinje cells via a presynaptic mechanism. Serotonergic neurons thus appear to play a role in the motor control through selective presynaptic facilitation that modulates the output from the cerebellar cortex.

There are no comments yet on this publication. Be the first to share your thoughts.