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Voltammetric behavior of 4-acetamidohippuric acid and 4-acetamidobenzoic acid on a disposable carbon electrode and their determination in human urine

Institute of Organic Chemistry and Biochemistry
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The voltammetric behavior of 4-aminobenzoic acid (PABA) and its acetylated metabolite on glassy carbon, carbon fiber or carbon paste electrodes was investigated in an aqueous supporting electrolyte. 4-Aminohippuric acid (4-AHA), 4-acetamidobenzoic acid (4-AMB) and 4-acetamidohippuric acid (4-Ac-AHA) can be separated on a capillary carbon paste electrode in 0.1 M lithium perchlorate. The oxidation potentials of PABA, 4-AHA, 4-AMB and 4-Ac-AHA were 0.70, 0.88, 1.06 and 1.10 V on capillary CPE, respectively. The electrooxidation process is used for simultaneous quantitative determination of acetylated metabolites in urine.

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