Affordable Access

Publisher Website

Effects of oxacillin and tetracycline on autolysis, autolysin processing andatltranscription inStaphylococcus aureus

Authors
Journal
International Journal of Antimicrobial Agents
0924-8579
Publisher
Elsevier
Publication Date
Volume
27
Issue
6
Identifiers
DOI: 10.1016/j.ijantimicag.2006.03.008
Keywords
  • Antibiotics
  • Atl
  • Autolysins
  • Autolysis
  • Staphylococcus Aureus
Disciplines
  • Biology
  • Philosophy

Abstract

Abstract Autolysins are peptidoglycan hydrolases involved in cell growth and cell lysis. Atl is an important autolysin of Staphylococcus aureus and is essential for penicillin-induced autolysis. The objective of our study was to examine the effect of oxacillin, chloramphenicol and tetracycline on autolysis, peptidoglycan hydrolase profiles and transcription of atl encoding the major S. aureus autolysin on cells grown in the presence of minimum inhibitory concentrations of the antibiotics. Growth of methicillin-susceptible strains in the presence of oxacillin led to increased autolysis, a loss of low molecular weight and a gain of high molecular weight peptidoglycan hydrolase bands suggesting altered proteolytic processing of peptidoglycan hydrolases, and a decrease in atl transcription. In contrast, growth in the presence of tetracycline led to a decrease in autolysis, an increase in atl transcription, and a drastic decrease in the protein concentration of freeze–thaw extracts obtained for peptidoglycan hydrolase analysis. Growth of methicillin-resistant strains in the presence of oxacillin had only moderate effects on autolysis and peptidoglycan hydrolase profiles.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Effects of oxacillin and tetracycline on autolysis...

on International Journal of Antim... June 2006

Tetracycline transport inStaphylococcus aureusH

on Biochimica et Biophysica Acta... Jan 01, 1969

Dichotomous selection of high-level oxacillin resi...

on International Journal of Antim... Jan 01, 2010

Detection of oxacillin-resistance inStaphylococcus...

on Diagnostic Microbiology and In... Jan 01, 1996
More articles like this..