Abstract With the combined advantages of high reliability, compact size and low electromagnetic interference, a high frequency operating thermoacoustic cooler system, i.e. a pulse tube cooler driven by a thermoacoustic heat engine, is quite promising for space applications. This article introduced a high frequency standing-wave thermoacoustic heat engine-driven pulse tube cooler system working around 300 Hz with axial length being 1.2 m. To improve the thermal efficiency of such system, an optimization has been carried out, both analytically and experimentally, by observing the influence of the dimensions of the stack, the hot buffer length and the acoustic pressure amplifier tube length. So far, a no-load temperature of 68.3 K has been obtained with 4.0 MPa helium and 750 W heating power. With 500 W heating power, a no-load temperature of 76.9 K and 0.2 W cooling power at 80 K have been achieved. Compared with former reports, the performance has been improved.