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Automated analysis of plasma and whole blood ascorbic acid

Authors
Journal
Clinical Biochemistry
0009-9120
Publisher
Elsevier
Publication Date
Volume
7
Identifiers
DOI: 10.1016/s0009-9120(74)91276-4

Abstract

An automated method using 2,6-dichloroindophenol is described for quantitatively determining ascorbic acid in plasma, whole blood and urine. Comparison with the 2,4-dinitrophenylhydrazine procedure for plasma ascorbic acid plus dehydroascorbic acid shows the former procedure to be as sensitive and easier to perform than the latter and reliable for assessing the vitamin C status of individuals. In a national sample of preschool children, plasma ascorbic acid determinations were obtained from 996 children. We demonstrated a progressive rise in plasma ascorbic acid levels with rise in intake; the rate of increase in plasma levels being greatest with intakes less than 90 mg/day (r=0.371, N=545), while a lower rate of increase was noted with intakes above 90 mg/day.

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