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Amino acid precursors for the detection of transketolase activity inEscherichia coliauxotrophs

Authors
Journal
Bioorganic & Medicinal Chemistry Letters
0960-894X
Publisher
Elsevier
Publication Date
Volume
19
Issue
14
Identifiers
DOI: 10.1016/j.bmcl.2009.04.111
Keywords
  • Transketolase
  • C–C Bond
  • Escherichia Coliauxotrophs
  • Selection Assay

Abstract

Abstract Probes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophs They combine the d- threo ketose moiety recognised by transketolase and the side chain of leucine or methionine. These compounds were donor substrates of yeast transketolase leading to the release of the corresponding α-hydroxyaldehydes which could be converted in E. coli by a cascade of reactions into leucine or methionine required for cellular growth.

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