Affordable Access

Publisher Website

A fast, sensitive determination of doxorubicin in rat plasma by solid-phase extraction and reversed-phase ion-pair chromatography

Authors
Journal
Analytica Chimica Acta
0003-2670
Publisher
Elsevier
Publication Date
Volume
217
Identifiers
DOI: 10.1016/s0003-2670(00)80394-3

Abstract

Abstract A very selective method is described for the determination of doxorubicin in rat plasma. Doxorubicin is extracted from the plasma on a pretreated octadecyl silane column and eluted with phosphate buffer pH 2.6/methanol (25/75, v/v) containing sodium 1-heptanesulfonate as ion-pairing agent. The extraction procedure is suitable for samples which contain doxorubicin encapsulated in liposomes if Triton X-100 is added. A portion of the evaporated eluate is used for high-performance reversed-phase chromatography with the same eluent and a fluorescence detector. Daunorubicin is used as internal standard. Extraction of doxorubicin from plasma is quantitative. The calibration graph is linear for 0.2-100 μg l −1 doxorubicin with a limit of detection of 0.2 μg l −1 for 0.5 ml of plasma. The relative standard error of estimate of the calibration was typically 3%.

There are no comments yet on this publication. Be the first to share your thoughts.