Affordable Access

Publisher Website

Effects of instability of samples during liquid scintillation counting on the results of ligand-binding studies by equilibrium dialysis

Authors
Journal
International Journal of Nuclear Medicine and Biology
0047-0740
Publisher
Elsevier
Publication Date
Volume
12
Issue
4
Identifiers
DOI: 10.1016/0047-0740(85)90184-6
Disciplines
  • Design

Abstract

Abstract In binding studies using equilibrium dialysis, mixing of the aqueous samples with scintillation cocktails designed to accommodate aqueous samples produced grossly erroneous binding data. The amount of [1,5- 14C]citrate apparently bound to rabbit muscle phosphofructokinase was a linear function of the citrate concentration, with no tendency to approach saturation, and highly variable. This problem was caused by very slow precipitation of citrate in the scintillation cocktail. Addition of a solubilizer stabilized the count rate during scintillation counting and resulted in acceptable binding curves.

There are no comments yet on this publication. Be the first to share your thoughts.