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The FlyBase database of the Drosophila genome projects and community literature

Nucleic Acids Research
Oxford University Press
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gkl842 61..65 NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins Kim D. Pruitt*, Tatiana Tatusova and Donna R. Maglott National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Rm 6An.12J, 45 Center Drive, Bethesda, MD 20892-6510, USA Received September 20, 2006; Revised and Accepted October 6, 2006 ABSTRACT NCBI’s reference sequence (RefSeq) database ( is a curated non-redundant collection of sequences represent- ing genomes, transcripts and proteins. The database includes 3774 organisms spanning prokaryotes, eukaryotes and viruses, and has records for 2 879 860 proteins (RefSeq release 19). RefSeq records integrate information from multiple sources, when additional data are available from those sources and therefore represent a current descrip- tion of the sequence and its features. Annotations include coding regions, conserved domains, tRNAs, sequence tagged sites (STS), variation, references, gene and protein product names, and database cross-references. Sequence is reviewed and fea- tures are added using a combined approach of collaboration and other input from the scientific community, prediction, propagation from GenBank and curation by NCBI staff. The format of all RefSeq records is validated, and an increasing number of tests are being applied to evaluate the quality of sequence and annotation, especially in the context of complete genomic sequence. INTRODUCTION RefSeq is a public database of nucleotide and protein sequences with feature and bibliographic annotation. The RefSeq database is built and distributed by the National Center for Biotechnology Information (NCBI), a division of the National Library of Medicine located at the US National Institutes of Health. NCBI makes RefSeq publicly available, at no cost, over the internet via Entrez query (1) and Basic Local Alignment Search Tool (BLAST) (2,3) programs, and incorporat

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