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Location of theabi,colandimmgenes on pHU011, a colicin Ib plasmid derivative

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
26
Identifiers
DOI: 10.1016/0378-1119(83)90201-9
Keywords
  • Recombinant Dna
  • Cleavage Map
  • Colicin Ib
  • Bacteriophage T5
  • Abortive Infection

Abstract

Abstract A cleavage site map of pHU011, a derivative of the colicin Ib plasmid containing the complete SalI-B fragment ligated to pBR322, has been determined. Sites of cleavage by PstI were determined using the Smith and Birnstiel [Nucl. Acids Res. 3 (1976) 2387–2398] method of mapping, whereas those for XbaI, XhoI, and HindIII were determined by double digestions or digestion of isolated fragments. In addition, the sites of the abi gene, which causes the abortive infection by T5 bacteriophage, and of the colicin ( col) gene have been determined. The results indicate that these genes are not contiguous.

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