Abstract Background To determine the effects of human amniotic fluid and membrane in the treatment of Achilles tendon ruptures, 72 tendons of 36 Wistar rats were injected with betamethasone sodium phosphate. Methods By the end of fourth week, both tendons were tenotomized and repaired, then the samples were divided into three groups. The first group was left untreated after suturing. Human amniotic fluid was injected to the second and amniotic fluid and membrane were both administered to the third group. Twenty-four tendons were scored at the end of the first week, and 24 at the end of the second week histopathologically, and 24 biomechanically at the end of the third week. Results There was a significant statistical difference only between the histopathological results of Groups 2 and 3 at the first week. Conclusions Human amniotic membrane and fluid do not add anything to the healing process of Achilles tendon ruptures in the early phase.