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Intramacrophage survival of uropathogenic Escherichia coli: Differences between diverse clinical isolates and between mouse and human macrophages

Authors
Publisher
Urban und Fischer Verlag
Publication Date
Keywords
  • Uropathogenic E
  • Coli (Upec) Are The Primary Cause Of Urinary Tract Infections
  • Recent Studies Have Demonstrated That Upec Can Invade And Replicate Within Epithelial Cells
  • Suggesting That This Bacterial Pathogen May Occupy An Intracellular Niche Within The Host
  • Given That Many Intracellular Pathogens Target Macrophages
  • We Assessed The Interactions Between Upec And Macrophages
  • Colonization Of The Mouse Bladder By Upec Strain Cft073 Resulted In Increased Expression Of Myeloid-
  • Consistent With The Recruitment Of Inflammatory Macrophages To The Site Of Infection
  • In In Vitro Assays
  • Cft073 Was Able To Survive Within Primary Mouse Bone Marrow-Derived Macrophages (Bmm) Up To 24H Post
  • Three Additional Well-Characterized Clinical Upec Isolates Associated With Distinct Uti Symptomatolo
  • Upec Strains Uti89 And Vr50
  • Originally Isolated From Patients With Cystitis And Asymptomatic Bacteriuria Respectively
  • Showed Elevated Bacterial Loads In Bmm At 24H Post-Infection As Compared To Cft073 And The Asymptoma
  • These Differences Did Not Correlate With Differential Effects On Macrophage Survival Or Initial Upta
  • E
  • Coli Uti89 Localized To A Lamp1+ Vesicular Compartment Within Bmm
  • In Contrast To Survival Within Mouse Bmm
  • Intracellular Bacterial Loads Of Vr50 Were Low In Both Human Monocyte-Derived Macrophages (Hmdm) And
  • Collectively
  • These Data Suggest That Some Upec Isolates May Subvert Macrophage Anti-Microbial Pathways
  • And That Host Species Differences May Impact On Intracellular Upec Survival
Disciplines
  • Biology

Abstract

Intramacrophage survival of uropathogenic Escherichia coli: Differences between diverse clinical isolates and between mouse and human macrophages - DTU Orbit (16/04/14) Intramacrophage survival of uropathogenic Escherichia coli: Differences between diverse clinical isolates and between mouse and human macrophages - DTU Orbit (16/04/14) Bokil, NJ, Totsika, M, Carey, AJ, Stacey, KJ, Hancock, V, Saunders, BM, Ravasi, T, Ulett, GC, Schembri, MA & Sweet, MJ 2011, 'Intramacrophage survival of uropathogenic Escherichia coli: Differences between diverse clinical isolates and between mouse and human macrophages' Immunobiology, vol 216, no. 11, pp. 1164-1171., 10.1016/j.imbio.2011.05.011

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