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Conjugal transfer of pAMβ1 inBacillus sphaericus1593

Authors
Journal
FEMS Microbiology Letters
0378-1097
Publisher
Oxford University Press
Publication Date
Keywords
  • Plasmid Transfer
  • Insecticidalbacillus
  • Filter Mating
Disciplines
  • Biology

Abstract

Abstract The plasmid pAMβ1 has been successfully transferred from Streptococcus faecalis to Bacillus sphaericus by the method of filter mating. Subsequently, transfer of this conjugative plasmid has also been demonstrated between derivatives of B. sphaericus 1593, though at reduced frequencies. The transfer process was determined to be unaffected by either endogenous restriction enzyme activity of the recipient or the presence of exogenous DNase.

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