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The colon as a site for vaccination: preliminary studies in mice using a nanoparticle vaccine.

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  • Biology

Abstract

The Colon as a Site for Vaccination: Preliminary Immunisation Studies in Mice using a Nanoparticle Vaccine The Colon as a Site for Vaccination: Preliminary Immunisation Studies in Mice using a Nanoparticle Vaccine Purpose: To establishdetermine whether the mouse colon ias a good site for vaccination, by quantification determination of the lymphoid tissue and of the immune responses generated following the use administration of a nanoparticle vaccine formulations (solution and nanoparticle suspension). Furthermore, to elucidate differences betweencompare the extent of, and to study differences in, o thef immune responses following vaccine delivery to the small intestine, colon, and rectum. To further compare the efficacy of the nanoparticle vaccine against an antigen solution with adjuvant. Methods. Lymphoid tissue in the colon and small intestine was ascertained quantifiedby following dissection and immersion of the gastrointestinal tract tissue in 10% v/v acetic acid (aq). Ovalbumin (model antigen) was encapsulated into poly (lactic co-glycolide) (PLGA) nanoparticles using a double solvent evaporation method. The nanoparticle vaccine, or antigen solution (with cholera toxin subunit B [mucosal adjuvant], or alum [intramuscular adjuvant]), were delivered to Balbc mice (i) orally, (ii) to the rectum (by pipette) (iii) to the colon (by flexible gavage tubing) and (iv) intramuscularly (IM). Three booster doses were given at two-weekly intervals. Animals were bleed before each booster, and antibodies collected from serum, from faecal material was collected to measure antibody levels. After sacrifice, antibodies were collected from the small intestine, colon, and vaginal mucosa were sampled. IgG levels in serum and IgA levels in the mucosa were determined by enzyme-linked immunosorbent assay (ELISA). Results. Significant amounts of lymphoid tissue were found in the mouse colon, comparable to the

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