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Assessment of nerve growth factor specific activity in tissue culture assay

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
117
Issue
2
Identifiers
DOI: 10.1016/0014-4827(78)90159-3
Disciplines
  • Biology

Abstract

Abstract 1. 1. The bioassay dose-response relationship is described for the nerve growth factor (NGF). A simple method for determination of the Biological Unit (BU) is described. 2. 2. It is proposed that the potency of any nerve growth promoting protein be expressed in terms of specific activity (BU/mg). If BU is defined in terms of the amount of protein which elicits a 3 + response, the specific activity is a dimensionless number.

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