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Production of alkali tolerant cellulase free xylanase in high levels by Bacillus pumilus SV-205

Authors
Journal
International Journal of Biological Macromolecules
0141-8130
Publisher
Elsevier
Publication Date
Volume
50
Issue
2
Identifiers
DOI: 10.1016/j.ijbiomac.2011.12.026
Keywords
  • Xylanase
  • Submerged Fermentation
  • Bacillus Pumilus
  • Wheat Bran
  • Alkali Stable
Disciplines
  • Biology

Abstract

Abstract The fermentation conditions were optimized for hyper production of xylanase from Bacillus pumilus SV-205. The bacterium secretes high levels (7382.7±1200IU/mL) of cellulase-free xylanase using wheat bran led to 21.63 fold increase in activity. A combination of yeast extract and peptone stimulated highest xylanase production (2448.0IU/mL) as compared to other combinations. The most important characteristic of the enzyme is its high pH stability (100%) over a broad pH range of 6–11 for 24h. Thermostability studies revealed that enzyme retained 65% activity after an incubation of 2h at 60°C. The level of production is remarkable as compared to earlier reports.

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