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Insecticidal activity of callus culture ofTagetes erecta

Authors
Journal
Fitoterapia
0367-326X
Publisher
Elsevier
Publication Date
Volume
75
Issue
1
Identifiers
DOI: 10.1016/j.fitote.2003.07.011
Keywords
  • Tagetes Erecta
  • Callus Culture
  • Pyrethrins
  • Ascorbic Acid

Abstract

Abstract The callus tissue of Tagetes erecta maintained on revised Murashige and Skoog's medium (RT) as static cultures showed the presence of insecticidal pyrethrins. The percentage of pyrethrins further increased by feeding the tissue with various concentrations of ascorbic acid. The maximum pyrethrin content (1.68%) was observed in 6 weeks old tissue grown on RT medium supplemented singly with 1000 mg/l of exogenous ascorbic acid. When pyrethrin mixture was screened against Tribolium spp. immediate ‘knock down’ effect was observed.

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