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Adenylate Cyclase Activity Not Found in Soybean Hypocotyl and Onion Meristem 1

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PMC
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  • Articles
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  • Biology
  • Chemistry

Abstract

Tissue, homogenates, and purified cell fractions prepared from hypocotyls of a dicot, soybean (Glycine max), and meristematic tissue of a monocot, onion (Allium cepa), were examined critically for evidence of adenylate cyclase activity. Three assay methods were used: chemical analysis, isotope dilution analysis, and enzyme cytochemistry. In both crude extracts or whole tissue, as well as purified membranes, with or without auxin, no adenylate cyclase was detected by any of the three methods.

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