Notifications of work accidents are instruments of epidemiological surveillance and evaluation of worker’s health conditions, occupational risks and work processes with which the worker is involved, enabling the implementation of strategies for health promotion. The purpose of this study was to analyze the work accidents with biological material that took place with nursing professionals in a Public Teaching Hospital, in the period from 1995 through 2000, to estimate risk indicators and to evaluate the impact of the implementations of the program of Work Accidents with Biological Materials (WABM) in the Institution. The data collection was made through the Report of Work Accidents and Notification of Work Accidents with Biological Material obtained from the competent divisions in the Institution. The statistical analysis used the Program Epi-Info and the Excel Electronic Plan. It was verified that out of the 421 reported accidents, 53,4% (225) referred to work accidents with biological material, with annual average risk coefficient of 5,9 per worker. The nursing assistants accounted for 90,2% (203) of the work accidents with biological material, with the highest annual average risk coefficient, that is 6,8. The mostly affected body parts were the hands, with a frequency of 82,8% of the cases and annual average risk coefficient of 4,9. The perforations represented a total of 70,7% (159) of the work accidents with biological material and 4,2 of annual average coefficient, associated mainly with the work of venous punches. In 11,6% of the work accidents with biological material there was occupational exposure to HIV serum positive patients. It was also verified the need to maintain educational actions for nursing professionals as well as of a review of the work processes to which these professionals are exposed, promoting the prevention of serious occupational diseases such the AIDS and Hepatitis B. There is also the need to guide the team as to the current legislation in order to improve the flow of notification for a better safety of the worker while providing him with the necessary legal support.