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Improved spectrophotometric methods for the assay of carbodiimides

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
114
Issue
1
Identifiers
DOI: 10.1016/0003-2697(81)90470-x
Keywords
  • Peptides
  • Amino Acids
  • And Amino Acid Derivatives
Disciplines
  • Biology
  • Physics

Abstract

Abstract Carbodiimides may be assayed by reacting with aqueous anilinium chloride and reading the absorbance at 230 nm after quenching in 1 m HCl. The basis of the assay is the formation of N-phenylguanidine which has a very large ultraviolet absorption; the limit of detection is 0.2 μ m. A second method of assay involves reacting the carbodiimide with acetic acid to yield anhydride which is assayed by the hydroxamic acid test with color development at 540 nm with ferric chloride. The limit of detection in the latter test is 20 μ m and provided suitable controls are used the methods are free from interference from proteins or nucleic acids.

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