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Low-volume jet injection for intradermal immunization in rabbits

Authors
Publisher
BioMed Central
Publication Date
Source
PMC
Keywords
  • Methodology Article

Abstract

1472-6750-2-10.fm ral BioMed CentBMC Biotechnology BMC Biotechnology 2002, 2 xMethodology article Low-volume jet injection for intradermal immunization in rabbits Shuxun Ren1,2, Minglin Li1,2, Joanne M Smith3, Louis J DeTolla3 and Priscilla A Furth*1,2,4 Address: 1Department of Oncology, Lombardi Cancer Center, Georgetown University, 3970 Reservoir Rd, NW, Washington, DC 20007, USA, 2Department of Medicine and Institute of Human Virology, University of Maryland Medical School, Baltimore, MD, 21201, USA, 3Comparative Medicine Program and Department of Pathology, University of Maryland Medical School, Baltimore, MD, 21201, USA and 4Department of Physiology, University of Maryland Medical School, Baltimore, MD, 21201, USA E-mail: Shuxun Ren - [email protected]; Minglin Li - [email protected]; Joanne M Smith - [email protected]; Louis J DeTolla - [email protected]; Priscilla A Furth* - [email protected] *Corresponding author Abstract Background: This study tested a low-volume (20–30 µl/20–30 µg DNA) jet injection method for intradermal delivery of a DNA vaccine. Jet injection offers the advantages of a needle-less system, low-cost, rapid preparation of the injected DNA solution, and a simple delivery system. More than one construct can be injected simultaneously and the method may be combined with adjuvants. Results: Low-volume jet injection targeted delivery of a DNA solution exclusively to the dermis and epidermis of rabbits. A three injection series of plasmid DNA, encoding the Hepatitis B Surface Antigen stimulated a humoral immune response in 2/5 rabbits. One rabbit developed a significant rise in antibody titer after 1 injection and one following 2 injections. There were no significant differences between jet injection and particle bombardment in the maximal antibody titers or number of injections before response. A three injection series of the same plasmid DNA by particle bombardment elicited a significant rise in antibody titer in 3/5 rabbits. O

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