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Morphological changes of temperature- and pH-stressedSalmonellafollowing exposure to cetylpyridinium chloride and nisin

Authors
Journal
LWT - Food Science and Technology
0023-6438
Publisher
Elsevier
Publication Date
Volume
39
Issue
10
Identifiers
DOI: 10.1016/j.lwt.2005.07.020
Keywords
  • Salmonella
  • Nisin
  • Cetylpyridinium Chloride
  • Outer Membrane

Abstract

Abstract The outer membrane of Gram-negative bacteria such as Salmonella, act as a permeability barrier, preventing nisin gaining access to the cytoplasmic membrane. If the outer membrane permeability is reduced, however, Gram-negative bacteria can show nisin sensitivity. In this study, temperature stresses (heating, chilling and freezing), pH stress (pH 4.5, 5.0, 6.0) and cetylpyridinium chloride (CPC)-nisin treatment were used to alter the outer membrane permeability of Salmonella, producing a loss of barrier function and reduced resistance to nisin. The morphological changes in Salmonella were examined using scanning electron microscopy. Temperature and pH-stressed S. typhimurium cells, untreated and treated with CPC-nisin had perturbed cell morphology, including apparent indentations and craters in the cell surfaces and collapsed amorphous bodies.

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