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The phorbol derivatives thymeleatoxin and 12-deoxyphorbol-13-O-phenylacetate-10-acetate cause translocation and down-regulation of multiple protein kinase C isozymes

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
319
Identifiers
DOI: 10.1016/0014-5793(93)80031-o
Keywords
  • Phorbol Ester
  • Protein Kinase C
  • Pc12 Cell
  • 12-Deoxyphorbol-13-O-Phenylacetate-20-Acetate
  • Thymeleatoxin
  • Phorbol 12-Myristate
  • 13-Acetate
Disciplines
  • Biology

Abstract

Abstract Phorbol esters such as phorbol 12-myristate,13-acetate (PMA) are potent activators of protein kinase C (PKC), and activate all PKC isozymes except ζ, and λ. Recently, 12-deoxyphorbol-13- O-phenylacetate-20-acetate (dPPA) and thymeleatoxin (Tx) were reported to selectively activate PKCβ 1 (dPPA) and PKCα, -β, and -γ (Tx), but not PKCδ or PKCϵ in vitro. We examined the ability of these phorbol derivatives to translocate and down-regulate PKC isozymes in intact cells. Our findings demonstrate that dPPA and Tx cause translocation and down-regulation of multiple PKC isozymes, including δ and ϵ.

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