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A Mammalian Cell Mutant with a Temperature-Sensitive Leucyl-Transfer RNA Synthetase

Authors
Publication Date
Source
PMC
Keywords
  • Biological Sciences: Cell Biology
Disciplines
  • Biology

Abstract

A cell mutant of the Chinese hamster ovary line, which is temperature sensitive for protein synthesis, is specifically defective in vivo in its ability to charge tRNA with leucine. Cytoplasmic extracts exhibited temperature-sensitive leucyl-tRNA synthetase activity. It is, therefore, highly likely that the mutant has a structural alteration in leucyl-tRNA synthetase. The low leakiness and low reversion rate of this mutant, combined with the specificity of the defect in its protein-synthesizing machinery, make it an appealing tool for investigating regulatory mechanisms in animal cells.

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