Affordable Access

Publisher Website

Antibody-induced redistribution of normal and tumor associated surface antigens

Experimental Cell Research
Publication Date
DOI: 10.1016/0014-4827(74)90234-1


Abstract Redistribution (capping) of normal and tumor-associated surface antigens was studied on murine and human cells by the indirect membrane immunofluorescence (MIF) technique. The capping of H-2 isoantigens was compared on normal mouse T-lymphocytes and on YAC cells, a Moloney leukemia virus (MLV) induced lymphoma. H-2 and Moloney virus induced cell surface antigen (MCSA) capping was compared on three YAC lines with different MCSA concentrations. H-2 and tumor-associated surface antigen capping was compared on two polyoma induced sarcoma lines and five methylcholanthrene induced sarcoma lines. In the human system, IgM-capping was compared on normal lymphocytes and on the Burkitt lymphoma derived Daudi line. Capping of HL-A and the Epstein-Barr virus (EBV) determined membrane antigen (MA) was compared on the Burkitt lymphoma derived line Maku and on EBV-superinfected Daudi cells. H-2 antigens on normal murine cells capped more promptly and on a larger fraction of the cell population on the various tumor cells. Surface associated IgM showed a better capping on normal lymphocytes than on Daudi cells. All tumor associated antigens except MCSA, showed good capping. MCSA was almost completely refractory to capping. Increasing concentrations of MCSA appeared to inhibit the capping of H-2 on the YAC sublines with different concentrations of MCSA. The polyoma induced ascites sarcoma (SEWA) did not cap either with regard to H-2 or the polyoma determined surface antigen.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Antibody-induced redistribution of normal and tumo...

on Experimental Cell Research October 1974

Antibody-induced redistribution of HL-A antigens a...

on European Journal of Immunology June 1972

Distribution and antibody-induced redistribution o...

on JNCI Journal of the National C... June 1975

Antibody-induced modulation and shedding of mammar...

on JNCI Journal of the National C... May 1976
More articles like this..