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Micro-electrophoretic determination of protein and protein synthesis in the 10−9to 10−7gram range

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
35
Identifiers
DOI: 10.1016/s0021-9673(01)82395-5
Disciplines
  • Biology

Abstract

Abstract A procedure is described of electrophoretic separation of 10 −7 to 10 −9 g of proteins on polyacrylamide gel in 200 μ or 400 μ diameter glass capillaries. Specific activities of tritium-labeled protein fractions are determined by a combustion technique in combination with interferometry. To be able to compare protein specific activities in identical structures of the same organism or of different animals, a procedure is described to correct for variations in the concentration of the precursor amino acid. An application of this method to a neurobiological problem is described.

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