Affordable Access

Publisher Website

Aberrant splicing of rat steroid 17α-hydroxylase transcripts

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
165
Issue
2
Identifiers
DOI: 10.1016/0378-1119(95)00536-f
Keywords
  • Exon Skipping
  • Polymerase Chain Reaction

Abstract

Abstract Polymerase chain reaction amplification of the cDNA encoding steroid 17α-hydroxylase (P450c17) demonstrated very low levels of this transcript and a shorter variant of still lower abundance in rat adrenals and brain. Sequence analysis of the two amplified products revealed that the shorter variant resulted from a deletion of the second exon which does not maintain the open reading frame, suggesting that faulty splicing gave rise to this minor species.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments