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Cytoplasmic pH, a key determinant of growth factor-induced DNA synthesis in quiescent fibroblasts

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
190
Issue
1
Identifiers
DOI: 10.1016/0014-5793(85)80439-7
Keywords
  • Na+/H+Antiporter
  • Phi
  • Fibroblast Mutant
  • Dna Synthesis
  • Growth Factor

Abstract

Abstract In response to growth factors, quiescent fibroblast mutants lacking Na +/H + exchange activity fail to elevate their cytoplasmic pH (pH i) and to reinitiate DNA synthesis at neutral and acidic pH o. A pH i threshold of ~7.2 exists, below which growth factors cannot set in motion the G 0 to S phase transition. Restoration of the pH i defect in mutant cells restores the wild-type phenotype. These findings, combined with the properties of another class of mutants able to grow at very low pH o, demonstrate that pH i modulated by growth factor activation of the Na +/H + antiporter, plays a determinant role in growth control.

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