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Analysis of ribonucleotides and deoxyribonucleotides using high-pressure liquid column chromatography

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
51
Identifiers
DOI: 10.1016/s0021-9673(01)96855-4

Abstract

Abstract Conditions for the separation of some mononucleotides, dinucleotides, nucleoside diphosphates and deoxymononucleotides on a pellicular anion-exchange column are described. These chromatographic systems allow quantification and identification of nucleotide materials in nanomole quantities and can be achieved within 100 min. The applicability of the present systems to the analysis of base composition of polynucleotides has been demonstrated by analyzing some oligonucleotides of known structure.

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