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Oropouche virus entry into HeLa cells involves clathrin and requires endosomal acidification

Authors
Journal
Virus Research
0168-1702
Publisher
Elsevier
Publication Date
Volume
138
Identifiers
DOI: 10.1016/j.virusres.2008.08.016
Keywords
  • Oropouche Virus
  • Clathrin
  • Endocytosis
  • Bunyaviridae

Abstract

Abstract Oropouche virus (ORO), family Bunyaviridae, is the second most frequent cause of arboviral febrile illness in Brazil. Studies were conducted to understand ORO entry in HeLa cells. Chlorpromazine inhibited early steps of ORO replication cycle, consistent with entry/uncoating. The data indicate that ORO enters HeLa cells by clathrin-coated vesicles, by a mechanism susceptible to endosomal acidification inhibitors. Transmission electron microscopy and immunofluorescence indicated that ORO associates with clathrin-coated pits and can be found in association with late endosomes in a time shorter than 1 h.

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