Abstract A concentric glucose/O 2 biofuel cell has been developed. The device is constituted of two carbon tubular electrodes, one in the other, and combines glucose electrooxidation at the anode and oxygen electroreduction at the cathode. The anodic catalyst is glucose oxidase co-immobilized with the mediator 8-hydroxyquinoline-5-sulfonic acid hydrate, and the cathodic catalyst is bilirubin oxidase co-immobilized with the mediator 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonate) diammonium salt. Both enzymes and mediators are entrapped at the surface of the tubular electrodes by an electrogenerated polypyrrole polymer. The originality of the concentric configuration is to compartmentalize the anode and cathode electrodes and to supply dissolved oxygen separate from the electrolyte in order to avoid secondary reactions. The dissolved oxygen circulates through the inside of the cathode tube and diffuses from the inner to the external surface of the tube to react directly with the immobilized bilirubin oxidase. The assembled biofuel cell is studied at 37 °C in phosphate buffer pH 7.4. We show the influence of the thickness of the polypyrrole polymer on the electrochemical activity of the biocathodes. We also demonstrate the effect of the chemical reticulation of the enzymes by glutaraldehyde within the polymer on the performances of the bioelectrodes. The maximum power density delivered by the assembled glucose/O 2 biofuel cell reaches 42 μW cm −2, evaluated from the geometric area of the electrodes, at a cell voltage of 0.30 V with 10 mM glucose. The results demonstrate that the concentric design of the BFC based on compartmented electrodes is a promising architecture for further development of micro electronic devices.