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3-Hydroxyphenylboronic Acid-Based Carbon Dot Sensors for Fructose Sensing

Authors
  • Crista, Diana M. A.1
  • Mello, Guilherme P. C.1
  • Shevchuk, Olena1
  • Sendão, Ricardo M. S.1
  • Simões, Eliana F. C.2
  • Leitão, João M. M.2
  • da Silva, Luís Pinto1, 3
  • Esteves da Silva, Joaquim C. G.1, 3
  • 1 Faculty of Sciences of University of Porto, Chemistry Research Unit (CIQUP), R. Campo Alegre 697, Porto, 4169-007, Portugal , Porto (Portugal)
  • 2 Faculdade de Farmácia da Universidade de Coimbra, Chemistry Research Unit (CIQUP), Pólo das Ciências da Saúde, Coimbra, 3000-548, Portugal , Coimbra (Portugal)
  • 3 Faculty of Sciences of University of Porto, LACOMEPHI, GreenUPorto, R. Campo Alegre 697, Porto, 4169-007, Portugal , Porto (Portugal)
Type
Published Article
Journal
Journal of Fluorescence
Publisher
Springer-Verlag
Publication Date
Jan 05, 2019
Volume
29
Issue
1
Pages
265–270
Identifiers
DOI: 10.1007/s10895-018-02336-2
Source
Springer Nature
Keywords
License
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Abstract

The selective fluorescence sensing of fructose was achieved by fluorescence quenching of the emission of hydrothermal-synthesized carbon quantum dots prepared by 3-hydroxyphenylboronic acid. Quantification of fructose was possible in aqueous solutions with pH of 9 (Limit of Detection LOD and Limit of Quantification LOQ of 2.04 and 6.12 mM), by quenching of the emission at 376 nm and excitation ~380 nm with a linearity range of 0–150 mM. A Stern-Volmer constant (KSV) of 2.11 × 10−2 mM−1 was obtained, while a fluorescent quantum yield of 31% was calculated. The sensitivity of this assay towards fructose was confirmed by comparison with other sugars (such as glucose, sucrose and lactose). Finally, the validity of the proposed assays was further demonstrated by performing recovery assays in different matrixes. Graphical Abstract

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